Fig. 1

Overexpression of MITF suppressed the expression of C/EBPβ and PPARγ in ovine primary preadipocytes. Primary preadipocytes were isolated from 20-day old small, fat-tailed sheep using the collagenase I digestion method. The cells were seeded in culture plates at a density of 5 × 10⁴ cells/cm² and cultured at 37 °C in a humidified atmosphere containing 5% CO₂. The cells were then induced to differentiate using the “cocktail” method. On days 0, 2, 4, 6 and 8 during adipogenic differentiation, the cells were collected and their total RNA and proteins were extracted. The expression levels of MITF, PU.1, C/EBPβ and PPARγ were detected via qPCR and western blotting analyses. a – The mRNA expression profiles of MITF, PU.1, C/EBPβ and PPARγ during adipogenic differentiation. The mRNA levels of β-tubulin on days 0, 2, 4, 6 and 8 were used as the reference for fold change calculation at each time point. b – The protein expression profiles of MITF, PU.1, C/EBPβ and PPARγ during adipogenic differentiation. c – Protein expression of C/EBPβ and PPARγ was suppressed by pcDNA-MITF transfection. d – Oil Red O staining for the ovine primary preadipocytes transfected with pcDNA-MITF on day 6 of differentiation