Fig. 2

Overexpression of MITF and its co-activator PU.1 inhibited adipogenesis of ovine primary preadipocytes. On reaching 80% confluence, the primary cells were transfected with pcDNA-MITF, pcDNA-PU.1, pcDNA-MITF and pcDNA-PU.1, or nothing. Then the cells were induced to differentiate. On day 6 of adipogenic differentiation, their total proteins were extracted. a – Expression of MITF, PU.1, C/EBPβ, PPARγ and aP2 after pcDNA-MITF and/or pcDNA-PU.1 transfection. b – Oil Red O staining for the ovine primary preadipocytes transfected with pcDNA-MITF and/or pcDNA-PU.1 on day 6 of differentiation. The scale bar is 200 μm. TG content change is shown in (c) (510 nm OD value) and (d) (μmol/ng protein) after primary preadipocytes were transfected with pcDNA-MITF and/or pcDNA-PU.1 on day 6 of differentiation. The same letters on the bar diagram indicated no significant differences between datasets; significant differences indicated by different letters. Significance was set at p < 0.05 (n = 3), *p < 0.05 vs. the control, ^# p < 0.05 vs. pc-MITF