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Fig. 3 | Cellular & Molecular Biology Letters

Fig. 3

From: MITF and PU.1 inhibit adipogenesis of ovine primary preadipocytes by restraining C/EBPβ

Fig. 3

Knockdown of MITF and PU.1 promoted adipogenesis of ovine primary preadipocytes. On reaching 80% confluence, the primary cells were transfected with MITF siRNA, PU.1 siRNA, MITF siRNA and PU.1 siRNA, or nothing. Then the cells were induced to differentiate. On day 6 of adipogenic differentiation, their total proteins were extracted. a – Expression of MITF, PU.1, C/EBPβ, PPARγ and aP2 after MITF siRNA and/or PU.1 siRNA transfection. b – Oil Red O staining for the ovine primary preadipocytes transfected with MITF siRNA and/or PU.1 siRNA on day 6 of differentiation; The scale bar is 200 μm. The bar diagram (bottom right) shows the OD value (510 nm) of the cell matrix containing ovine primary preadipocytes transfected with MITF siRNA and/or PU.1 siRNA on day 6 of differentiation. TG content change is shown in (c) (510 nm OD value) and (d) (μmol/ng protein) after the primary preadipocytes were transfected with MITF siRNA and/or PU.1 siRNA on day 6 of differentiation. The same letters on the bar diagram indicated no significant differences between datasets; significant differences indicated by different letters. Significance was set at p < 0.05 (n = 3), *p < 0.05 vs. control, # p < 0.05 vs. siMITF

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