Fig. 2

Nomarski contrast images of cells during parallel isolations from the same single trabeculectomy biopsy using 5% FGF-EMEM and 10% DMEM. a – Healthy outgrowth of only fibroblasts from the explant when 5% FGF-EMEM was used. b – Healthy outgrowth of a co-culture of predominant epithelium-derived cells (white arrows) and single HTFs (black arrows) from the explant when 10% DMEM was used. c – Monolayer of HTFs 7 days after passage 1 when 5% FGF-EMEM was used. d – Few epithelium-derived cells (white arrows) and single fibroblast (black arrow) 7 days after passage 1 when 10% DMEM was used. e –Few epithelium-derived cells (white arrows) and fibroblasts (black arrows) 13 days after passage 1 when 10% DMEM was used. f – Initial recovery of HTF culture 4 days after medium change to 5% FGF-EMEM (black arrows indicate fibroblasts, white arrows indicate epithelium-derived cells); scale bar = 75 μm, magnification 200x