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Fig. 3 | Cellular & Molecular Biology Letters

Fig. 3

From: A simple and effective protocol for fast isolation of human Tenon’s fibroblasts from a single trabeculectomy biopsy – a comparison of cell behaviour in different culture media

Fig. 3

Fluorescence laser scanning microscope images of HTF cultures obtained 48 h after passage 2. a, c – HTFs isolated using 5% FGF-EMEM. b, d – HTFs isolated using 10% DMEM with medium change to 5% FGF-EMEM on the approx. 26th day. a, b have a scale bar = 75 μm, magnification 200x, while c, d have a scale bar = 37 μm, magnification 400x. Green fluorescence – vimentin filaments; red fluorescence – F-actin filaments; blue fluorescence – nuclei

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