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Fig. 5 | Cellular & Molecular Biology Letters

Fig. 5

From: A simple and effective protocol for fast isolation of human Tenon’s fibroblasts from a single trabeculectomy biopsy – a comparison of cell behaviour in different culture media

Fig. 5

Morphology and size of HTFs during parallel cultivation in 5% FGF-EMEM and 10% DMEM. a, b – Fluorescence laser scanning microscope images obtained upon cytoskeleton staining of HTFs (white arrows indicate fibroblasts just after division); scale bar = 75 μm, magnification 200x; red fluorescence – F-actin filaments; blue fluorescence – nuclei. c – HTF size determined using ImageJ software; results are expressed as means ± SD. *Significantly lower spreading area (p < 0.0001) compared to 10% DMEM according to the unpaired t-test (GraphPad Prism 5, Version 5.03 Software)

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Cellular & Molecular Biology Letters

ISSN: 1689-1392

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