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Fig. 7 | Cellular & Molecular Biology Letters

Fig. 7

From: A simple and effective protocol for fast isolation of human Tenon’s fibroblasts from a single trabeculectomy biopsy – a comparison of cell behaviour in different culture media

Fig. 7

Type I collagen production by HTFs during parallel cultivation in 5% FGF-EMEM and 10% DMEM. a, b – Fluorescence laser scanning microscope images obtained upon immunofluorescent staining of Col I; scale bar = 37 μm, magnification 400x; red fluorescence – Col I, blue fluorescence – nuclei. c – Intensity of the Col I fluorescence (normalized per 103 cells) measured using microplate reader. Results are expressed as means ± SD. *Significantly lower intensity of Col I fluorescence (p = 0.0007) compared to 10% DMEM according to the unpaired t-test (GraphPad Prism 5, Version 5.03 Software)

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