Fig. 2From: PPARγ agonist through the terminal differentiation phase is essential for adipogenic differentiation of fetal ovine preadipocytesEffect of rosiglitazone supplementation and prolonged adipogenic cocktail exposure on ovine fetal and adult preadipocyte (oPADs) differentiation. Quantification of ORO positive area (mean ± SEM; a), representative ORO stain images of fetal (B1 (only insulin supplementation in days 3 to 8); B2 (optimized medium)) fetal and adult oPADs (B3; optimized medium) after 8 days of differentiation (b), and differentiation medium details (c). Scale bar: 50 μm. BM: basal medium, Dex: dexamethasone, DM: differentiation medium, Ins: insulin, oPADs: ovine preadipocytes, Rosi: rosiglitazone. Three fetal oPADs and three adult oPADs primary cells (passage 3) from three different fetuses and three different adult sheep, respectively, were used. Asterisks represent significant differences (P < 0.05)Back to article page