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Fig. 2 | Cellular & Molecular Biology Letters

Fig. 2

From: PPARγ agonist through the terminal differentiation phase is essential for adipogenic differentiation of fetal ovine preadipocytes

Fig. 2

Effect of rosiglitazone supplementation and prolonged adipogenic cocktail exposure on ovine fetal and adult preadipocyte (oPADs) differentiation. Quantification of ORO positive area (mean ± SEM; a), representative ORO stain images of fetal (B1 (only insulin supplementation in days 3 to 8); B2 (optimized medium)) fetal and adult oPADs (B3; optimized medium) after 8 days of differentiation (b), and differentiation medium details (c). Scale bar: 50 μm. BM: basal medium, Dex: dexamethasone, DM: differentiation medium, Ins: insulin, oPADs: ovine preadipocytes, Rosi: rosiglitazone. Three fetal oPADs and three adult oPADs primary cells (passage 3) from three different fetuses and three different adult sheep, respectively, were used. Asterisks represent significant differences (P < 0.05)

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