Fig. 4
Sirt1/FoxO1 overexpression neutralized myoblast inhibition by H19. a The overexpression efficiency of H19. Cells without H19 transfection (scrambled) were the negative control and wild-type cells were the blank control (control). The protein levels of MyoG, MyHC, Sirt1 and FoxO1 in bovine tibia skeletal muscle (sk-muscle) satellite cells (b and d) and C2C12 cells (c and e) with H19 overexpression. f, g RT-PCR results for bovine sk-muscle satellite cells (f) and C2C12 cells (g) with H19 overexpression. h, i Real-time qPCR results for bovine sk-muscle satellite cells (h) and C2C12 cells (i) with H19 overexpression. The cells transfected with the vector without H19 (scrambled) were the control and GAPDH was the internal control. The protein and mRNA abundance was normalized to the GAPDH gene, n = 3, *p < 0.05, **p < 0.01