Fig. 5

SDAD1 promotes the proliferation, migration and invasion of colon cancer cells. a The expression of SDAD1 in 27 pairs of colon cancer and adjacent non-tumor tissues was determined using quantitative RT-PCR. b The MTT assay was performed on SW480 and HCT-116 cells transfected with SDAD1 overexpression plasmid. c A colony formation assay was performed on SDAD1 overexpression plasmid-transfected SW480 and HCT-116 cells. d FACS was performed to analyze the apoptosis of SW480 cells when SDAD1 was overexpressed. e The effect of SDAD1 transfection on the migration/invasion of SW480 and HCT-116 cells was determined using Transwell plates. f Western blot was used to measure the protein levels of the EMT protein makers in SW480 cells transfected with SDAD1 overexpression plasmid. *p < 0.05, **p < 0.01, ****p < 0.0001