Fig. 1

SFRP2 increased the expression of phosphorylated β-catenin in SCAPs. a Real-time RT-PCR results confirmed the overexpression of SFRP2 via lentivirus infection in SCAPs. GAPDH was used as an internal control. b Western blot results showed that the overexpression of SFRP2 increased the expression of phosphorylated β-catenin and phosphorylated GSK-3β. Beta-actin was used as an internal control. c Quantitative analysis of phosphorylated β-catenin based on western blot results. d Real-time RT-PCR detection of SFRP2 expression after SCAPs were infected with short hairpin RNAs (shRNA) that silenced SFRP2 (SFRP2sh) compared with scrambled shRNA (Scramsh). GAPDH was used as an internal control. e Western blot results showed that depletion of SFRP2 downregulated the expression of phosphorylated β-catenin. f Quantitative analysis of phosphorylated β-catenin based on western blot results. Beta-catenin was used as an internal control. Student’s t test was performed to determine statistical significance. All error bars represent SD (n = 3). **p ≤ 0.01