Fig. 1

Cell survival and apoptosis analyses after up- and downregulation of ADAM23 in ReNcell VM cells. a Western blot analyses of: endogenous ADAM23 in the control groups with pCA-GFP or pGS-GFP plasmid-transfected cells revealed by human ADAM23 antibody (lanes in pCA-GFP and pGS-GFP); upregulated ectopic ADAM23 in pCA-A23-transfected cells revealed by chicken ADAM23 (lanes in pCA-A23); and downregulated endogenous ADAM23 in pGS-A23-transfected cells (lanes in pGS-A23) revealed by human ADAM23 antibody. The images were taken on proliferation day (d0), and 3 (d3) or 7 days (d7) after the initiation of differentiation. GAPDH was used as a loading control. b The cell viability in the pGS-A23-transfected group decreased when compared to the control pGS-A23 and pCA-A23 groups on proliferation day (d0) and 3 (d3) and 7 days (d7) after the initiation of differentiation, as revealed with the CASY assay. pCA-GFP and pGS-GFP were used as controls. Data are presented as mean ± SEM from at least three independent experiments. *p < 0.05 and **p < 0.01. c The percentages of apoptotic cells were measured 2 days after transfection with pCA-A23 and pGS-A23 plasmids on d0 and 3 days (d3) after the initiation of differentiation. pCA-GFP was used as a control. Data are presented as mean ± SEM from at least three independent experiments. *p < 0.05