Fig. 2

Upregulation of ADAM23 increases neuronal differentiation, as shown by flow cytometry measurements. The dot plots with a forward scatter (FSC-H) vs. fluorescence (FL4-H) show the total cell and fluorescence-positive cell numbers after transfection with pCA-GFP control (a–c) and pCA-A23 (d–f) 3 days (d3) and 7 days (d7) after the initiation of differentiation. The percentages of Tuj-1-positive cells were also measured (g). Note that the Tuj-1-positive cells were marked as red in the dot plots, as indicated by R-1 region. Overexpression of pCA-A23 obviously increased the number of red dots. Data are presented as mean ± SEM from at least three independent experiments. *p < 0.05