Fig. 1

Expression profile of the members of the NR4A sub-family in BeWo cells treated with forskolin. BeWo cells were treated with forskolin (25 μM) for various time periods, followed by analysis of NR4A sub-family members at the mRNA and protein levels. a – Transcript profiles of Nor-1, Nurr-1 and Nur-77 in BeWo cells at 0, 1, 2, 6, 24 and 48 h of forskolin treatment. Relative expression was normalized with 18S rRNA. Data are expressed as means ± s.e.m. of three independent experiments performed in triplicate. b – Densitometeric plots of Nor-1, Nurr-1 and Nur-77 using GAPDH as a loading control. Data are represented as means ± s.e.m. of at least three experiments. Representative blots for the same are also shown. p ≤ 0.05 is considered statistically significant