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Fig. 2 | Cellular & Molecular Biology Letters

Fig. 2

From: Relevance of the NR4A sub-family of nuclear orphan receptors in trophoblastic BeWo cell differentiation

Fig. 2

Effect of Nor-1, Nurr-1 and Nur-77 silencing on forskolin-mediated BeWo cell fusion and hCG secretion. BeWo cell knockdown for Nor-1, Nurr-1 or Nur-77 were made using siRNA as described in the Methods section. The efficacy of silencing of Nor-1, Nurr-1 or Nur-77 transcripts was confirmed via qRT-PCR using specific primers and was studied after 0, 2 and 48 h of forskolin (25 μM) treatment in control siRNA-transfected and Nor-1-, Nurr-1- or Nur-77-silenced BeWo cells. a, b and c – Comparisons of the transcript levels of Nor-1, Nurr-1 and Nur-77, respectively, in the control siRNA-transfected and silenced cells. Each bar represents relative expression after normalization with 18S rRNA and is expressed as means ± s.e.m. of three independent experiments performed in triplicate. The effect of Nor-1, Nurr-1 or Nur-77 silencing on forskolin-mediated BeWo cell fusion was studied at 48 h using desmoplakin I + II staining and hCG secretion was studied with ELISA. d – The fold change in forskolin-mediated BeWo cell fusion in Nor-1-, Nurr-1- or Nur-77-silenced cells and control siRNA-transfected cells. Values are shown as the means ± s.e.m. of three independent experiments. Representative images of desmoplakin I + II staining (green) are appended alongside. The scale bar represents 20 μm. e – hCG secreted by control and Nor-1-, Nurr-1- or Nur-77-silenced cells in response to forskolin treatment at 48 h and represented as means ± s.e.m. of three independent experiments performed in duplicate. p ≤ 0.05 is considered statistically significant

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