Table 1 Primer sequences used in quantitative RT-PCR
Gene | Primer | Annealing temperature |
|---|---|---|
Nor-1 | F 5′-CTCTAAAGACGGAACCGCCA-3′ R 5′-ATGAGGGCCTGGAGGGTATC-3′ | 60 °C |
Nurr-1 | F 5′-CTATTCCAGGTTCCAGGCGA-3′ R 5′-CATTCCCCAAAGCCACGAAC-3′ | 60 °C |
Nur-77 | F 5′-CACGGAGCGCTTAAGAGGAG-3′ R 5′-AGGCAGGTCTTGGCAACAAT-3′ | 60 °C |
18S | F 5′-GGAGAGGGAGCCTGAGAAAC-3′ R 5′-CCTCCAATGGATCCTCGTTA-3’ | 60 °C |