Fig. 5

The effect of LPS on IkB phophorylation and degradation and flavonoids on IkB degradation. The time course of IκBα (a) and IκBβ degradation (b) and IκBα phosphorylation (c). Cells were pre-treated with flavonoids (10 μM) for 30 min and later exposed to LPS (1 μg/ml) for 15 min, to then be lysed. d – H9c2 cells were incubated with flavonoids (10 μM) for 30 min, then stimulated with LPS (1 μg/ml) for 60 min. Whole cell extract was processed via sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS-PAGE), and the membranes were blotted with IκB-specific phosphoantibodies. The results are representative of three separate experiments. Densitometric analysis represents the means ± SEM. *p < 0.05 vs. untreated basal value