Fig. 6

Effect of LPS and flavonoids on COX-2 expression. a – H9c2 cells were incubated with LPS at different doses. b – H9c2 cells were pretreated with flavonoids (10 μM) for 1 h and then incubated with LPS (1 μg/ml) for 12 h. c – H9c2 cells were pretreated with different inhibitors and then treated with LPS (1 μg/ml). The lysates of the cells were processed via sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS-PAGE), and the membranes were blotted with COX-2-specific antibodies. The results are representative of three separate experiments. Tubulin was used as an internal control for western blot analysis. Statistical significance was determined with a two-way ANOVA test. *p < 0.05 vs. untreated group