Fig. 1

HIWI2 is aberrantly expressed in retinoblastoma. a, b – Real-time PCR shows the expression of HIWI2 transcripts in ARPE19, HeLa and Y79 cell lines. β-actin was used for normalization and fold changes in expression are indicated. c – Western blot shows the expression of HIWI2 in protein lysates of ARPE19 and HeLa. β-ACTIN was used for normalization and the fold changes are indicated. The bar graph represents the quantification of the western blot image representing the fold change in the expression of HIWI2 in ARPE19 and HeLa cells. d – Western blot shows the expression of HIWI2 in protein lysates of HeLa and Y79 cell lines. β-ACTIN was used for normalization and the fold changes are indicated. The bar graph represents the quantification of the western blot image representing the fold change of HIWI2 in HeLa and Y79 cell lines. Student’s t-test was used for statistical analysis. *p < 0.05 and **p < 0.01 were considered statistically significant