Fig. 2

OTX2 is downregulated upon HIWI2 knockdown. a – Representative western blot image showing HIWI2 expression level in Si-HIWI2 Y79 cells. β-ACTIN was used for normalization and the fold changes are indicated. b – Representative proteome profiler array blot used for screening Si-Control and Si-HIWI2 Y79 protein lysates. c – Fold changes in the expressions of the proteins that are altered in Si-HIWI2 Y79 cells obtained from quantification of the array, are represented in the graph. d – Representative western blot image showing OTX2 expression level in Si-HIWI2 Y79 cells. β-ACTIN was used for normalization and the fold changes are indicated. e – Real-time PCR results indicating the fold changes in the levels of Otx2 transcript in Si-HIWI2 Y79 cells. f – Real-time PCR results showing the reduced expression of Otx2 transcripts in Si-HIWI2 ARPE19 cells. Student’s t-test was used for statistical analysis. *p < 0.05 and **p < 0.01 were considered statistically significant