Fig. 2
From: The novel protein C3orf43 accelerates hepatocyte proliferation
Preparation of virus and identification of C3orf43 overexpression and knockdown. a 293 T cells transfected with pCDH-C3 were observed with fluorescence microscopy. The bright field is to the left and the fluorescence image is to the right. b BRL-3A cells infected with lentiviral particles were observed with fluorescence microscopy. The bright field is to the left and the fluorescence image is to the right. c The C3orf43 content in the pCDH-C3 group was evaluated via western blot. d and e The expression of C3orf43 was determined using qRT-PCR and western blot. NC, siR1, siR2 and siR3 represent the cells treated with negative siRNA, siR1-C3orf43, siR2-C3orf43 or siR3-C3orf43, respectively. The experiments were performed three times with three replicates in each case. The data are shown as the means ± SD. Representative images are shown. *p < 0.05, **p < 0.01 difference relative to the control