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Fig. 1 | Cellular & Molecular Biology Letters

Fig. 1

From: miR-200b downregulates CFTR during hypoxia in human lung epithelial cells

Fig. 1

Regulation of CFTR during hypoxia in human lung epithelial cells, Calu3 cells (left panels) and 16HBE14o- cells (right panels). a CFTR mRNA is reduced during hypoxia. CFTR mRNA levels were monitored in qRT-PCR experiments. The results from 3 independent experiments (n = 12) are plotted normalized to 18S rRNA levels and expressed as a fold-change over the normoxic control. b Hypoxia sequentially increases HIF-1α protein levels and reduces CFTR protein levels. Protein expression levels of were monitored with SDS-PAGE and Western Blot and normalized to β-actin levels. Two individual samples (4 μg of total protein per lane) were tested for each time point and the experiments were repeated twice. Error bars represent standard deviations. Significant changes (P < 0.05) are marked with an asterisk

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