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Fig. 2 | Cellular & Molecular Biology Letters

Fig. 2

From: miR-200b downregulates CFTR during hypoxia in human lung epithelial cells

Fig. 2

Downregulation of CFTR expression during hypoxia is HIF-1 dependent and relies on both the 5′ and 3′ UTRs of CFTR mRNA. a Calu3, 16HBE14o- and HeLa WT CFTR cells were treated with hypoxia mimetics (500 μM DMOG for 12 h (light grey) and 200 μM CoCl2 for 12 h (dark grey)) and the mRNA levels were monitored in qRT-PCR experiments. CFTR mRNA levels from 2 independent experiments (n = 8) are plotted relative to 18S rRNA levels and expressed as a fold change over the untreated control. b Calu3 cells were transfected with 5′UTR CFTR luciferase reporter (white), 3′UTR CFTR luciferase reporter (light grey) and co-transfected with both 5′UTR and 3′UTR CFTR luciferase reporters (dark grey) and treated with hypoxia mimetics (500 μM DMOG or 200 μM CoCl2 for 12 h) and the luciferase activity was monitored. These reporters were normalized to internal controls (Renilla) firefly luciferase activities from 2 independent experiments (n = 6) and plotted and expressed as a fold-change over non-treated control. Error bars represent standard deviations. Significant changes (P < 0.05) are marked with an asterisk

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