Fig. 6

Effects of the BM-MNC-conditioned medium on fibroblast migration in the wound scratch assay. a – A confluent monolayer of fibroblasts was scratched and the conditioned medium obtained in a standard (S+) or hypoxic (H+) atmosphere was then added. Images were captured at 0, 12, 24, 36, 48 and 56 h (data not shown) and compared with their control in the same atmosphere (S- or H-). Scratch closure was measured as the width of each scratch using Image J software (version 1.45). b – Each time point was normalized to the image perimeter on day 0 and reported as percent closure. The results are expressed as means ± SEM (n = 6). *p < 0.05 compared with the control in the same atmosphere (S- or H-). **p < 0.001 compared with the control in the same atmosphere (S- or H-). ***p < 0.01 compared with the control in the standard atmosphere (S-). ^# p < 0.01 compared with S+