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Fig. 3 | Cellular & Molecular Biology Letters

Fig. 3

From: IFN-λ1 in CHO cells: its expression and biological activity

Fig. 3

SDS-PAGE and western blot analysis of IFN-λ1. a – Cation exchange chromatography elution profile. Lanes 1, 2, and 3 correspond to the three phases of the elution peak: rise, plateau and fall. Lane 4 shows the unbound protein. b – The corresponding western blot of the gel in A. c – Blue Sepharose 6 Fast Flow affinity chromatography purification results. Lane 1 shows the target protein eluted by buffer A, which containing 50% ethylene glycol. Lane 2 shows the protein in elution peak 1. d – Sephacryl S-100 gel purification results. Lane 1 shows the purified IFN-λ1 and lane 2 shows the solution before purification. All the samples were separated using 12% SDS-PAGE and visualized using Coomassie blue staining. M: protein molecular weight marker

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