Fig. 6
From: Collagen-derived dipeptide prolyl-hydroxyproline promotes osteogenic differentiation through Foxg1
Effect of Pro-Hyp on the protein expression levels of Foxg1, Runx2, osterix, Col1α1, osteocalcin and Foxo1 in Foxg1-knockdown cells. Foxg1 was knocked down in MC3T3-E1 cells, which were then cultured with or without 0.1 mM Pro-Hyp for 4 days. After incubation, cell lysates were collected and the expression levels of Foxg1, Runx2, osterix, Col1α1, osteocalcin and Foxo1 were analyzed via western blotting. The data are representative of 3 independent experiments