Fig. 2From: Ectopic expression of TWIST1 upregulates the stemness marker OCT4 in the esophageal squamous cell carcinoma cell line KYSE30Enforced expression of TWIST1 upregulates OCT4 mRNA expression in KYSE30 cells. a – TWIST1 is overexpressed nine-fold in GFP-hTWIST1 compared to the control. This caused a statistically significant eight-fold increase in OCT4 mRNA expression. b – Phase contrast and fluorescent microscope images of GFP-hTWIST1 and control cells. The phase contrast images show the cells after 24 h and the fluorescent images are after 48 hBack to article page