Fig. 2

Expression of mdr1 and p-gp in HEp-2 cells after overexpression of c-fos. a Fluorescence microscopy examination of the efficiency of transfecting the c-fos overexpression plasmid into HEp-2 cells (named c-fos WT). The negative control (NC) was HEp-2 cells transfected with an empty vector. The scale bar represents 100 μm. b, c Real-time PCR results showing the expression of c-fos and mdr1 in HEp-2 cells after the overexpression of c-fos. d Western blot results showing the expression level of c-fos and p-gp in HEp-2 cells after the overexpression of c-fos. e, f The statistical quantification analyses of c-fos and p-gp protein levels in c-fos overexpression HEp-2cells.Data are shown as the means ±SD. **p < 0.01, ***p < 0.001