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Fig. 4 | Cellular & Molecular Biology Letters

Fig. 4

From: Downregulation of microRNA-448 inhibits IL-1β-induced cartilage degradation in human chondrocytes via upregulation of matrilin-3

Fig. 4

Matrilin-3 was a direct target of miR-448. Chondrocytes were treated with 5 ng/ml IL-1β for 24 h after transfection with miR-448 mimic or inhibitor. a The mRNA and protein levels of matrilin-3 were respectively determined using quantitative real-time PCR and western blot. Matrilin-3 expression was normalized to GAPDH. b Schematic representation of matrilin-3 3’UTRs showing putative miRNA target site. c The analysis of the relative luciferase activities of matrilin-3-WT and matrilin-3-MUT. All data are presented as means ± SEM, n = 4. *p < 0.05, **p < 0.01 vs. control; ##p < 0.01, ###p < 0.001 vs. vehicle + IL-1β or miR-NC or anti-miR-NC

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