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Fig. 4 | Cellular & Molecular Biology Letters

Fig. 4

From: Arsenic trioxide induces apoptosis and the formation of reactive oxygen species in rat glioma cells

Fig. 4

Effects of As2O3 on ROS generation in C6 and 9 L cells as measured using DCFH-DA. a C6 cells without exposure to 5 μmol/l As2O3 showed a mean fluorescence intensity of 7.58, while those with exposure for 24, 48 and 72 h showed 200.37, 344.80 and 501.74, respectively. 9 L cells without exposure to 5 μmol/l As2O3 showed a mean fluorescence intensity of 3.01, while those with exposure for 24, 48 and 72 h showed 180.27, 248.32 and 485.90, respectively. b Quantification data of ROS. Cells pretreated with NAC significantly inhibited the 24 h As2O3 increase in ROS levels. *p < 0.05 vs. control, #p < 0.05 vs. 24 h

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