Fig. 2

The inhibition of PDGF-C results in HT1080 cell growth retardation. a The PDGF-R inhibitor suppresses the growth of HT1080 cells. HT1080 cells were cultured for 3 days with the indicated concentrations of AG1296 and subjected to the WST assay. b and c PDGF-C knockdown reduces the growth of HT1080 cells. b HT1080/hPDGFC(1304) cells were treated with (+) or without (−) doxycycline (Dox) for 3 days and subjected to the WST assay. Notably, the expression of PDGF-C was repressed in the absence of doxycycline in HT1080/hPDGFC(1304) cells. c HT1080 cells were transfected with 500 nM of PDGF-C-specific siRNA (siPDGF-C) or negative control siRNA (siGFP), and subjected to the WST assay after a 3-day culture. d PDGF-C knockdown inhibits anchorage-independent growth of HT1080 cells. HT1080/hPDGFC(1304) cells were subjected to the soft agar assay with or without Dox for 2 weeks. In all the experiments, the average result for the control samples was set to 100%, and the bars and error bars respectively represent the means and standard deviations (n = 3). Statistical analysis was performed using the two-tailed paired t test. Statistical significance is defined as p < 0.05. The raw data are shown in Additional file 1: Figure S2