Fig. 3

PDGF-C is expressed in self-renewing ES cells and downregulated during ES cell differentiation. a Reduction of PDGF-C mRNA expression by LIF depletion. After culture with LIF for 3 days (+LIF) or without LIF for 6 days (−LIF), the expression levels of PDGF-C, Oct3/4 and Rex1 in the E14 ES cells were examined using RT-PCR. b The reduction in PDGF-C mRNA expression by Oct3/4 depletion. ZHBTc4 ES cells were cultured with (+) or without (−) doxycycline (Dox) for 2 days. Expression of the indicated genes was examined by RT-PCR. c Reduction of PDGF-C mRNA expression by the expression of a dominant-negative mutant of STAT3. After transfection with pCAG-IP (control) or pCAG-dnSTAT3-IP (dnSTAT3), E14 ES cells were cultured with puromycin for 2 days. Expression of the indicated genes was examined using RT-PCR. d Expression of PDGF receptors in ES cells. Total RNA was prepared from E14 ES cells, C2C12 myoblasts and NIH3T3 fibroblasts and subjected to RT-PCR. In all experiments, the results shown are representative of three independent experiments