Fig. 4From: GSK-3β-mediated regulation of cadmium-induced cell death and survivalCadmium-induced apoptosis was increased by GSK-3β knockdown. SH-SY5Y cells were transfected with GSK-3β siRNA, and total proteins were extracted and separated using SDS-PAGE. a – Representative FACS data for apoptosis are shown. b – Western blot analysis was performed using anti-GSK-3β antibodies (inset). Apoptotic cells were counted using the annexin V assay. The data are expressed as the means ± SEM of the percentage of apoptotic cells from at least three independent experiments. ***p < 0.001Back to article page