Fig. 3
From: HIF-1α promotes the migration and invasion of hepatocellular carcinoma cells via the IL-8–NF-κB axis
Overexpression of exogenous IL-8 restored the migration and invasion that had been attenuated by NF-κB pathway inhibition. a HepG2 cell migration aftersiHIF-1α treatment and subsequent IL-8 treatment under conditions of normoxia and hypoxia. b HepG2 cell invasion after siHIF-1α treatment and subsequent IL-8 treatment under conditions of normoxia and hypoxia. c Western blot assay of HIF-1α, IL-8, pNF-κB and NF-κB after siIL-8 treatment. GAPDH was used as the loading control. d HepG2 cell migration was decreased by PDTC when IL-8 was overexpressed. e HepG2 cell invasion was decreased by PDTC when IL-8 was overexpressed. The results represent three independent experiments. Con.: control; PDTC-: treated without PDTC; PDTC+: treated with PDTC