Fig. 6

Confocal microscope images of the tubulin structure in HeLa cells using immunofluorescence staining. a and b Medium-propagated cells (a) and vehicle-treated control cells (b) showed normal and intact microtubules. c HeLa cells treated with ESE-16 revealed a decrease in cell density and a complete loss and disruption of the microtubule network. d A HeLa cell in prophase with an intact microtubule network. e A HeLa cell after 24 h exposure to ESE-16, revealing an increased number of cells in metaphase with disintegrated microtubules