Fig. 2

Characterization of differentiated MSCs pellets in chondrogenic differentiation medium supplemented with 5% PL. a Three-dimensional round tissue pellet after 3 weeks of culture in differentiation medium. b Acridine orange staining and the distribution of the cells in the pellets i) Sections of undifferentiated pellets (negative control), image at 150× magnification. ii) Sections of differentiated pellets, image at 150× magnification. iii) Sections of chicken articular cartilage (positive control), image at 600× magnification. c Alician blue staining. i) Sections of undifferentiated pellets (negative control), image at 150× magnification. ii) Sections of differentiated pellets, image at 150× magnification. iii) Sections of chicken articular cartilage, image at 300× magnification. d 2% agarose gel electrophoresis for amplified transcripts of SOX9 and GAPDH. i) Amplified transcripts in undifferentiated pellets. ii) Amplified transcripts in differentiated pellets. The data are representative from one out of three donors tested (n = 3)