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Fig. 3 | Cellular & Molecular Biology Letters

Fig. 3

From: Mitochonic acid 5 activates the MAPK–ERK–yap signaling pathways to protect mouse microglial BV-2 cells against TNFα-induced apoptosis via increased Bnip3-related mitophagy

Fig. 3

MA-5 enhanced mitophagy via Bnip3. a through f The proteins were isolated and analyzed via western blotting. The mitophagy parameters were evaluated in control cells (Ctrl), and in cells treated with 10 ng/ml TNFα (TNFα), 10 ng/ml TNFα and 5 μM MA-5 (TNFα+MA-5), or 10 ng/ml TNFα and 5 μM MA-5 after silencing of Bnip3 with an siRNA (TNFα+MA-5 + si-Bnip3). g and h An immunofluorescence assay of mitophagy via mitochondria and lysosome co-staining in cells treated with 10 ng/ml TNFα (TNFα), or 10 ng/ml TNFα and 5 μM MA-5 (TNFα+MA-5). i The caspase-9 activity with MA-5 treatment (TNFα+MA-5) or Bnip3 knockdown (TNFα+MA-5 + si-Bnip3). *p < 0.05 vs. Ctrl group; #p < 0.05 vs. TNFα group; †p < 0.05 vs. TNFα+MA-5 group

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Cellular & Molecular Biology Letters

ISSN: 1689-1392

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