Fig. 6

PKC activation reversed the inhibition of norpurpureine on agonist-stimulated platelet aggregation. Typical traces of platelet aggregation monitored by changes in the light transmission signal over time are shown in a platelet-rich plasma (PRP) and b washed platelets (WP). Platelets were exposed to norpurpureine inhibitory action for 10 min, then challenged with ADP and collagen (Col) or thrombin (Thr) followed by PMA, as indicated. PMA (control) in each platelet preparation is shown. These original tracings are representative of three experiments done independently for each agonist. Quantification of the data in (a) and (b) are shown in (c). Each data point is the mean ± SD (n = 3), p < 0.001(***) vs. PMA in each platelet preparation