Fig. 4

Osthole suppresses IL-1β-induced expression of MMPs and pro-inflammatory factors in SW982 cells. SW982 cells were cultured with 10 ng/ml IL-1β and different concentrations of osthole for 48 h. a Total proteins (MMP-1, MMP-3 and MMP-13) were extracted from the SW982 cells and analyzed by western blot. β-Actin was chosen as a loading control. b Culture supernatants were collected, and the cytokine concentrations of IL-6 (B) and TNF-α (c) in the medium were measured by ELISA kits. **p < 0.01 compared with the normal group, ^#p < 0.05 compared with the 10 ng/ml IL-1β group