Skip to main content
Fig. 2 | Cellular & Molecular Biology Letters

Fig. 2

From: MicroRNA-495 inhibits the high glucose-induced inflammation, differentiation and extracellular matrix accumulation of cardiac fibroblasts through downregulation of NOD1

Fig. 2

Effects of miR-495 on pro-inflammatory cytokines in human CFs incubated in high glucose. CFs were transfected with miR-495 inhibitor or mimic for 48 h, and then treated with 5.5 or 25 mM glucose for 24 h. HG + miR-NC indicates CFs transfected with the miR-495 inhibitor before treatment with high glucose; HG + miR-495 mimic indicates CFs transfected with the miR-495 mimic before treatment with high glucose. a The mRNA expressions of NOS2 and COX2 were determined via quantitative RT-PCR. b The secreted NOS2 and COX2 proteins in the supernatants of CFs were detected using ELISA assays. The data shown are means ± SEM (n = 4). **p < 0.01 vs. control; ##p < 0.01 vs. vehicle + high glucose. The vehicle is transfection reagent alone

Back to article page