Fig. 3

Effects of miR-495 on high glucose-induced differentiation in human CFs. CFs were transfected with miR-495 inhibitor or mimic for 48 h, and then treated with 5.5 or 25 mM glucose for 24 h. HG + miR-NC indicates CFs transfected with the miR-495 inhibitor before treatment with high glucose; HG + miR-495 mimic indicates CFs transfected with the miR-495 mimic before treatment with high glucose. a and b The mRNA and protein expressions of α-SMA were respectively determined via quantitative RT-PCR and western blot. The data shown are means ± SEM (n = 6). **p < 0.01, ***p < 0.001 vs. control; ^##p < 0.01 vs. vehicle + high glucose. The vehicle is transfection reagent alone