Fig. 5

NOD1 was a direct target of miR-495. Human CFs were transfected with miR-495 inhibitor or mimic for 48 h. a Schematic representation of NOD1 3’UTRs showing putative miRNA target site. b The analysis of the relative luciferase activities of NOD1-WT, NOD1-MUT in CFs. c The mRNA and protein expressions of NOD1 were respectively determined via quantitative RT-PCR and western blot. NOD1 expression was normalized to GAPDH. All data are presented as means ± SEM (n = 6). ^##p < 0.01, ***p < 0.001 vs. miR-NC or miR-NC inhibitor