Fig. 1

Anti-β2GPI/β2GPI complex induced the release of platelet-derived microparticles (MPs) and miRNAs. a Representative image of isolated MPs observed via transmission electron microscopy. Three MPs are indicated with arrows. b and c The amount of MPs with thrombin, the antibody complex or the control (TBS or NC) in the P1 area was detected using flow cytometry. MP gates were constructed via flow cytometry using size-calibrated beads. d and e The level of miR-96 (d) or miR-26a (e) in platelet-derived MPs was measured using quantitative RT-PCR. Cel-miR-46* was used as the internal control. f The relative levels of miR-26b-3p, −26b-5p and − 96-3p in the p-MPs were detected using quantitative RT-PCR. The results are presented as mean ± SD of four independent experiments (*p < 0.05, **p < 0.01 vs. TBS group)