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Fig. 2 | Cellular & Molecular Biology Letters

Fig. 2

From: Laminopathies: what can humans learn from fruit flies

Fig. 2

a The salivary gland nuclei of 3rd instar larvae with polytene chromosomes are the best known feature of the Drosophila model system. Dissected salivary glands and prepared nuclei are shown, stained for lamin Dm (green) with rabbit affinity purified anti-lamin Dm antibody and lamin C (red) with the ALC28.12 monoclonal antibody. DNA is counterstained with DAPI. b A dissected 3rd instar larvae thick specimen stained for lamin Dm (green) and lamin C (red) with antibodies as in section A. Only differentiated cells express lamin C. Both diploid and polyploid nuclei are visible. c Salivary gland nuclei of 3rd instar larvae, with polytene chromosomes, dissected from a fly strain overexpressing EGFP-lamin Dm (green) under the control of the Act5C-Gal4 driver. Note the increased thickness of the nuclear lamina and its irregular structure, composed of aggregates, cisterns and invaginations. d Nuclei of nurse cells of egg chambers and follicular cells stained for lamin Dm (red) and membranes (green). Drosophila egg chambers contain nurse cells with polyploid nuclei (up to 1024 N) and are frequently used as controls for chromatin organization in polyploid cells without polytene chromosome structure. Please note the diploid oocyte nuclei in which chromatin fills only part of the cell nucleus

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