Skip to main content
Fig. 6 | Cellular & Molecular Biology Letters

Fig. 6

From: MicroRNA-488 inhibits tongue squamous carcinoma cell invasion and EMT by directly targeting ATF3

Fig. 6

Overexpression of ATF3 partially promoted cell proliferation, invasion and EMT in miR-488-overexpressing TSCC cells. TCA8113 cells were transfected with either miR-488 mimic with or without pcDNA-ATF3 vector. a The mRNA and protein expressions of ATF3 were determined via quantitative RT-PCR and western blot assays, respectively. b Invasion was assessed using a transwell assay. c The protein expressions of MMP-2, MMP-7 MMP-9, TIMP-1 and TIMP-2 were assessed using ELISA assays. d The expressions of E-cadherin, N-cadherin and vimentin were determined via quantitative RT-PCR and western blot assays. All data are presented as means ± SEM, n = 6. #p < 0.05, ##p < 0.01, ###p < 0.001 vs. pcDNA3.1 + miR-448 mimic

Back to article page