Fig. 5

ClC-2 deficiency blocked cerebrovascular remodeling. a C57BL/6 mice were injected with ClC-2-shRNA adenovirus (sh-ClC-2) or Lacz adenovirus before AngII infusion. Representative images of hematoxylin and eosin staining of basilar arteries. b Vascular remodeling was evaluated based on the cross-sectional area (CSA). c Representative images of immunofluorescence staining for α-SMA expression. d Bar graph showing the the relative fluorescence density values for α-SMA expression. e through h β-catenin (e) and GSK-3β (f) phosphorylation, and survivin (g) and cyclin D1 (h) protein expression were determined via western blotting. *p < 0.01, **p < 0.01 vs. Lacz, ^##p < 0.01 vs. AngII+Lacz, n = 8 mice in each group