Fig. 1
From: DSPP-MMP20 gene silencing downregulates cancer stem cell markers in human oral cancer cells
Lentiviral mediated knockdown of DSPP (shDSPP), MMP20 (shMMP20) and DSPP+MMP20 (shDM) in OSC2 cells, and generation of stable DSPP / MMP-20 / DSPP+MMP20 silenced OSC2 cell lines. Protein expression levels of DSPP and MMP20 (a) are presented as relative fold change levels in knockdowns (shDSPP, shMMP20 and shDM) compared to scramble-control (shC) (b). Among the different stable lines selected, L4 exhibited the maximum knockdown (73%) for DSPP-silencing, L3 for MMP20 (74%) and L5 for DSPP+MMP20 silencing (73 and 75% for DSPP and MMP20, respectively), as compared to shC at the protein level