Fig. 3
From: DSPP-MMP20 gene silencing downregulates cancer stem cell markers in human oral cancer cells
WB analysis of OCSC makers following DSPP-MMP20 silencing and treatment with Cisplatin. a Total protein levels; b ABCG2; c ALDH1; d BMI1; e CD44; f LGR4; g CD133; h Podoplanin. Treatment with cisplatin was for 72 h at concentration of 5, 10, or 50 μΜ. Normalization of protein level was with β-actin. Y-(vertical) axis of histograms represent expression level for each protein relative to β-actin. OSC2 = parent (untreated) cells; shC = control (scramble sequence) cells; shD = DSPP silenced cells; shM = MMP20 silenced cells; shDM = combined DSPP-MMP20 silenced cells. *p < 0.05 compared with ShC. Experiments were performed in triplicate