Fig. 1

HIF-1α is associated with the migration and invasion of human hepatocarcinoma (HCC)cells by regulating IL-8 expression under conditions of hypoxia. a Relative HIF-1α mRNA levels in human HCC cell lines and the normal liver cell line WRL68. n = 3; *p < 0.05. b HIF-1α and IL-8 protein levelsin HCC cell lines and the normal liver cell line WRL68. GAPDH was used as the loading control. c Relative IL-8 mRNA levels in human HCC cell lines and the normal liver cell line WRL68. n = 3; *p < 0.05. d The efficiency of HIF-1α knockdown in HCC cell lines (left panel) and the effect on IL-8 expression (right panel) were evaluated using quantitative RT-PCR. e Western blot analysis of HIF-1α and IL-8 protein expression in HCC cell lines treated with siHIF-1α. GAPDH was used as a control. n = 3; *p < 0.05