Fig. 2

Exogenous IL-8 restored the migration and invasion that had been attenuated by Akt pathway inhibition. a HIF-1α antibody was used for ChIP, followed by quantitative PCR with specific primer pairs for the IL-8 promoter (n = 3; *p < 0.05) in two HCC cell lines under hypoxia. b Relative Hep3B cell migration aftersiHIF-1α treatment and IL-8 addback treatment under normoxia and hypoxia. The numbers of migrated cells from 5 independent experiments were analyzed. Scale bar: 200 μm (bottom panel) c Relative Hep3B cell invasion aftersiHIF-1α treatment and IL-8 addback treatment under normoxia and hypoxia. d Western blot assay of HIF-1α, IL-8, pAkt and the Akt response to siHIF-1α treatment. GAPDH was used as the loading control. e Relative Hep3B cell migration showed a decrease when cells overexpressing HIF-1αwere treated with LY294002. f – Relative Hep3B cell invasion showed a decrease when cells overexpressing HIF-1α were treated with LY294002. All results represent three independent experiments. LY294002-: treated without LY294002; LY294002+: treated with LY294002